SKP2 (Ab-64) Antibody

Catalog Number:

21700

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q13309
NCBI Protein#: NP_005974.2.

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.62~66 (P-E-S-P-P) derived from Human SKP2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total SKP2 protein.

Applications:

WB

Background:

 Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

 

1)Tedesco D., Lukas J., Reed S.I.Genes Dev. 16:2946-2957(2002)  

2)Mendez J., Zou-Yang X.H., Kim S.Y., Hidaka M., Tansey W.P., Stillman B.Mol. Cell 9:481-491(2002)

3)Li X., Zhao Q., Liao R., Sun P., Wu X.J. Biol. Chem. 278:30854-30858(2003)

References:

appl_detail:

 Western blotting: 1:500~1:1000

Datasheet PDF