Product Datasheet  
SKP2 (Ab-64) Antibody  
Catalog Number: 21700  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q13309
    NCBI Protein#: NP_005974.2.
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C
  • Immunogen：  
  • Peptide sequence around aa.62~66 (P-E-S-P-P) derived from Human SKP2.
  • reactivity：  
  • Hu
  • appl_detail：  

  •  Western blotting: 1:500~1:1000

  • other_names：  
  • FBXL1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total SKP2 protein.
  • Applications：  
  • WB
  • Background：  

  •  Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

     
    1)Tedesco D., Lukas J., Reed S.I.Genes Dev. 16:2946-2957(2002)  
    2)Mendez J., Zou-Yang X.H., Kim S.Y., Hidaka M., Tansey W.P., Stillman B.Mol. Cell 9:481-491(2002)
    3)Li X., Zhao Q., Liao R., Sun P., Wu X.J. Biol. Chem. 278:30854-30858(2003)



 
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