SPARC Antibody

Catalog Number:

21633

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P09486
NCBI Protein: NP_003109.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total SPARC protein.

Applications:

WB

Background:

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990)
Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988)
Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995)

References:

appl_detail:

Predicted MW: 42kd
Western blotting: 1:500~1:1000

Datasheet PDF