Product Datasheet  
SPARC Antibody  
Catalog Number: 21633  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P09486
    NCBI Protein: NP_003109.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 42kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • ON; BM-40;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total SPARC protein.
  • Applications：  
  • WB
  • Background：  
  • Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

    Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990)
    Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988)
    Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995)



 
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