MEK5 Antibody

Catalog Number:

21559

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q62862
NCBI Protein: NP_001029159.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa. 311-315(K-T-Y-V-G ) derived from Rat MEK5.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide

Specificity/Sensitivity:

The antibody detects endogenous level of total MEK5 protein.

Applications:

WB

Background:

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Han, J., et al. 1996. J. Biol. Chem. 271: 2886-2891.
Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926.
Tournier, C., et al. 1997. Proc. Natl. Acad. Sci. USA94: 7337-7442.
Holland, P.M., et al. 1997. J. Biol. Chem. 272: 24994-24998.

References:

appl_detail:

Predicted MW: 49kd
Western blotting: 1:500

Datasheet PDF