Product Datasheet  
MEK5 Antibody  
Catalog Number: 21559  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q62862
    NCBI Protein: NP_001029159.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa. 311-315(K-T-Y-V-G ) derived from Rat MEK5.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 49kd
    Western blotting: 1:500
  • other_names：  
  • MAP kinase kinase 5; Map2k5; MAPKK 5; MAPK/ERK kinase 5;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
  • Specificity：  
  • The antibody detects endogenous level of total MEK5 protein.
  • Applications：  
  • WB
  • Background：  
  • A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

    Han, J., et al. 1996. J. Biol. Chem. 271: 2886-2891.
    Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926.
    Tournier, C., et al. 1997. Proc. Natl. Acad. Sci. USA94: 7337-7442.
    Holland, P.M., et al. 1997. J. Biol. Chem. 272: 24994-24998.



 
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