Glutamine Synthetase Antibody

Catalog Number:

21650

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P15104
NCBI Protein: NP_001028216.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.365~369(G-D-E-P-F) derived from Human Glutamine Synthetase

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Glutamine Synthetase protein.

Applications:

WB

Background:

This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner . Essential for proliferation of fetal skin fibroblasts. "Glutamine synthetase isolated from human brain: octameric structure and homology of partial primary structure with human liver glutamine synthetase."
Boksha I.S., Schonfeld H.J., Langen H. Biokhimiia 67:1012-1020(2002) "Glutamine synthetase is essential for proliferation of fetal skin fibroblasts."
Vermeulen T., Goerg B., Vogl T. Arch. Biochem. Biophys. 478:96-102(2008)

References:

appl_detail:

Predicted MW: 42kd
Western blotting: 1:500~1:1000
 

Datasheet PDF