PI3 Kinase p85/p55(Ab-467/199) Antibody

Catalog Number:

21508

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P27986
Gene ID:5295

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.465~469(L-Y-E-E-Y) derived from Human PI3 Kinase p85/p55.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total PI3 Kinase p85/p55 protein.

Applications:

IHC

Background:

PI3K binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Stoyanov, B. et al. (1995) Science 269, 690-3.
Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.

References:

appl_detail:

Predicted MW: 55, 85kd
Immunohistochemistry: 1:50~1:100

Datasheet PDF