synaptophysin 2 Antibody

Catalog Number:

21452

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P22831
NCBI Protein: NP_076464.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.257~261(G-P-T-S-F)derived from Rat synaptophysin 2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of total synaptophysin 2 protein.

Applications:

WB

Background:

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).

Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
Valtorta, F. et al. (2004) Bioessays 26, 445-53.
Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
Bonanomi, D. et al. (2007) Biochem J 404, 525-34.

References:

appl_detail:

Predicted MW: 37kd
Western blotting: 1:500~1:1000

Datasheet PDF