MAP2K4 Antibody

Catalog Number:

21441

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P45985
NCBI Protein: NP_003001.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.352~356(E-S-K-R-P)derived from Human MAP2K4.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MAP2K4 protein.

Applications:

WB

Background:

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
Sanchez, I. et al. (1994) Nature 372, 794-798.
Yan, M. et al. (1994) Nature 372, 798-800.
Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

References:

appl_detail:

Predicted MW: 44kd
Western blotting: 1:500~1:1000

Datasheet PDF