Product Datasheet  
MAP2K4 Antibody  
Catalog Number: 21441  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P45985
    NCBI Protein: NP_003001.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.352~356(E-S-K-R-P)derived from Human MAP2K4.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 44kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • JNKK1; MEK4; MKK4; PRKMK4; SERK1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous levels of total MAP2K4 protein.
  • Applications：  
  • WB
  • Background：  
  • SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

    Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
    Sanchez, I. et al. (1994) Nature 372, 794-798.
    Yan, M. et al. (1994) Nature 372, 798-800.
    Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
    Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.



 
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