MDM2 Antibody

Catalog Number:

21436

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q00987
NCBI Protein: NP_002383.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.18~22(Q-I-P-A-S) derived from Human MDM2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MDM2 protein.

Applications:

WB

Background:

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .

Haupt, Y. et al. (1997) Nature 387, 296-299.
Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

References:

appl_detail:

Predicted MW: 90kd
Western blotting: 1:500~1:1000

Datasheet PDF