Product Datasheet  
MDM2 Antibody  
Catalog Number: 21436  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q00987
    NCBI Protein: NP_002383.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.18~22(Q-I-P-A-S) derived from Human MDM2.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 90kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • HDMX; hdm2;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous levels of total MDM2 protein.
  • Applications：  
  • WB
  • Background：  
  • MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .

    Haupt, Y. et al. (1997) Nature 387, 296-299.
    Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
    Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
    Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.



 
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