Caspase 9 Antibody

Catalog Number:

21422

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P55211
NCBI Protein: NP_001220.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Caspase 9 protein.

Applications:

WB

Background:

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
Li, P. et al. (1997) Cell 91, 479-489.

References:

appl_detail:

Predicted MW: 47 37 35kd
Western blotting: 1:1000

Datasheet PDF