Keratin 18(Ab-33) Antibody

Catalog Number:

21306

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P05783
NCBI Protein: NP_000215.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.31~35 (A-A-S-V-Y) derived from Human Keratin 18 (CK18).

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Keratin 18 protein.

Applications:

WB IHC

Background:

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
Moll, R. et al. (1982) Cell 31, 11-24.

References:

appl_detail:

Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF