IKK-b(Ab-199) Antibody

Catalog Number:

21305

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O14920
NCBI Protein: NP_001547.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.197~201 (Q-K-Y-T-V) derived from Human IKK-b.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total IKK-bprotein.

Applications:

WB IF

Background:

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.

Chen RA, et al. PLoS Pathog. 2008 Feb 8;4(2):e22
Yen CJ,et al. Cancer Res. 2008 Apr 15;68(8):2632-40

References:

appl_detail:

Predicted MW: 87kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Datasheet PDF