H2B Antibody

Catalog Number:

21417

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P33778
NCBI Protein: NP_003519.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.41~45 (Y-V-Y-K-V) derived from Human H2B.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total H2B protein.

Applications:

WB

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Borchert GM,et al. (2010) PLoS One.5(7):e11641.
Pennini ME,et al.(2010) PLoS Pathog.6(7):e1000995.
Lu C,et al. (2010) Sci China Life Sci.53(6):663-8.

References:

appl_detail:

Predicted MW: 14kd
Western blotting: 1:500~1:1000

Datasheet PDF