4E-BP1 (Ab-37) Antibody

Catalog Number:

21215

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13541
NCBI Protein: NP_004086.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.35~39 (S-T-T-P-G) derived from Human 4E-BP1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total 4E-BP1 protein.

Applications:

WB IHC

Background:

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Gingras AC, et al. Genes Dev Nov. 2005 Feb 15.
Mothe-Satney I, et al.(2000) J Biol Chem:15(21): 2852-64.
Gingras AC, et al. (1999) Genes Dev:13(11): 1422-37.
Lal L, et al.(2005) Blood;105(4): 1669-7.

References:

appl_detail:

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF