Product Datasheet  
4E-BP1 (Ab-37) Antibody  
Catalog Number: 21215  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q13541
    NCBI Protein: NP_004086.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.35~39 (S-T-T-P-G) derived from Human 4E-BP1.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Predicted MW: 18kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • EIF4EBP1; PHAS-1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total 4E-BP1 protein.
  • Applications：  
  • WB IHC
  • Background：  

  • 4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

    Gingras AC, et al. Genes Dev Nov. 2005 Feb 15.
    Mothe-Satney I, et al.(2000) J Biol Chem:15(21): 2852-64.
    Gingras AC, et al. (1999) Genes Dev:13(11): 1422-37.
    Lal L, et al.(2005) Blood;105(4): 1669-7.




 
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