HSF1(Ab-307) Antibody

Catalog Number:

21206

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q00613
NCBI Protein: NP_005517.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa. 305~309 (P-Q-S-P-R) derived from Human HSF1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total HSF1 protein.

Applications:

WB IHC

Background:

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4
Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026
Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.

References:

appl_detail:

Predicted MW: 82kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF