Product Datasheet  
HSF1(Ab-307) Antibody  
Catalog Number: 21206  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species:  
  • Rabbit
  • Amount:  
  • 100μgμg
  • Swiss-Prot No.:  
  • Swiss-Prot: Q00613
    NCBI Protein: NP_005517.1
  • Form of Antibody:  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage:  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen:  
  • Peptide sequence around aa. 305~309 (P-Q-S-P-R) derived from Human HSF1.
  • reactivity:  
  • Hu
  • appl_detail:  
  • Predicted MW: 82kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names:  
  • HSTF 1
  • Purification:  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity:  
  • The antibody detects endogenous level of total HSF1 protein.
  • Applications:  
  • WB IHC
  • Background:  
  • DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

    Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4
    Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026
    Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.



 
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