Product Datasheet
HSF1(Ab-307) Antibody
Catalog Number: 21206
Technical:tech@swbio.com
Information:info@swbio.com
Description
- Swiss-Prot No.:
- Swiss-Prot: Q00613
NCBI Protein: NP_005517.1
- Form of Antibody:
- Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Storage:
- Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
- Immunogen:
- Peptide sequence around aa. 305~309 (P-Q-S-P-R) derived from Human HSF1.
- appl_detail:
- Predicted MW: 82kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
- Purification:
- Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
- Specificity:
- The antibody detects endogenous level of total HSF1 protein.
- Background:
- DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4
Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026
Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.
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