STAT6(Ab-641) Antibody

Catalog Number:

21050

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P42226
NCBI Protein: NP_003144.3

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.639~643 (R-G-Y-V-P) derived from Human STAT6.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total STAT6 protein.

Applications:

WB IHC

Background:

STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. By screening an embryonic lung fibroblast cDNA library with a wildtype STAT6 probe, 2 variant cDNAs were identified, which were termed STAT6B and STAT6C, encoding an N-terminal 110-amino acid truncation and a 27-amino acid deletion in the SH2 domain, respectively. All the three variants are ubiquitously expressed with STAT6B expression greatest in spleen and STAT6C expression greatest in lung. STAT6B resembles an attenuated STAT6, but that STAT6C inhibits IL-4-mediated mitogenesis and cell surface antigen expression, and is not tyrosine phosphorylated

Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738.
Malabarba M G, et al. (1996) Biochem. J. 319:865-872.
Hou J, et al. (1994) Science. 265:1701-1706.
Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343.
Takeda K, et al. (1996) Nature. 380: 627-630.

References:

appl_detail:

Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

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