CREB (Phospho-Thr100) Antibody

Catalog Number:

12133

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P16220;
NCBI Gene#:1385

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 100 (S-G-T(p)-Q-I) derived from Human CREB.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of CREB only when phosphorylated at threonine 100.

Applications:

WB

Background:

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF