Smad1 (Phospho-Ser187) Antibody

Catalog Number:

12074

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15797;
NCBI Gene#: 4086;
NCBI Protein#: NP_001003688.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 187 (P-H-S(p)-P-N) derived from Human Smad1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Smad1 only when phosphorylated at Serine 187.

Applications:

WB

Background:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.

References:

appl_detail:

Predicted MW: 55,60kd
Western blotting: 1:500~1:1000

Datasheet PDF