Histone H4 (Acetyl-Lys5) Antibody

Catalog Number:

12070

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P62805;
NCBI Gene#: 8359;
NCBI Protein#: NP_003529.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around acetylation site of Lysine 5(S-G-R(p)-G-K) derived from Human Histone H4.

Purification:

Antibodies were produced by immunizing rabbits with synthetic acetylpeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific acetylpeptide. Non-acetyl specific antibodies were removed by chromatogramphy using

Specificity/Sensitivity:

The antibody detects endogenous level of Histone H4 only when acetylated at Lysine 5.

Applications:

WB

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

References:

appl_detail:

Predicted MW: 11kd
Western blotting: 1:500~1:1000

Datasheet PDF