iNOS (Phospho-Tyr151) Antibody

Catalog Number:

12067

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P35228;
NCBI Gene#: 4843;
NCBI Protein#: NP_000616.3

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine 151 (Q-Y-Y(p)-G-S) derived from Human iNOS.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of iNOS only when phosphorylated at Tyrosine 151.

Applications:

WB

Background:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

References:

appl_detail:

Predicted MW: 130kd
Western blotting: 1:500~1:1000

Datasheet PDF