Product Datasheet  
iNOS (Phospho-Tyr151) Antibody  
Catalog Number: 12067  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P35228;
    NCBI Gene#: 4843;
    NCBI Protein#: NP_000616.3
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Tyrosine 151 (Q-Y-Y(p)-G-S) derived from Human iNOS.
  • reactivity：  
  • Hu Rt
  • appl_detail：  
  • Predicted MW: 130kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • NOS, INOS, NOS2A, HEP-NOS
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of iNOS only when phosphorylated at Tyrosine 151.
  • Applications：  
  • WB
  • Background：  
  • Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.



 
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