B-RAF (Phospho-Ser446) Antibody

Catalog Number:

12064

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P15056;
NCBI Gene#: 673;
NCBI Protein#: NP_004324.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 446 (R-D-S(p)-S-D) derived from Human B-RAF.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of B-RAF only when phosphorylated at Serine 446.

Applications:

WB

Background:

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

References:

appl_detail:

Predicted MW: 110kd
Western blotting: 1:500~1:1000

Datasheet PDF