p47 phox (Phospho-Ser359) Antibody

Catalog Number:

12054

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P14598;
NCBI Gene#: 653361;
NCBI Protein#: NP_000256.4

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 359 (Q-R-S(p)-K-P) derived from Human p47 phox.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of p47 phox only when phosphorylated at Serine 359.

Applications:

WB

Background:

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

References:

appl_detail:

Predicted MW: 45kd
Western blotting: 1:500~1:1000

Datasheet PDF