Product Datasheet  
p47 phox (Phospho-Ser359) Antibody  
Catalog Number: 12054  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species:  
  • Rabbit
  • Amount:  
  • 100μgμg
  • Swiss-Prot No.:  
  • Swiss-Prot#: P14598;
    NCBI Gene#: 653361;
    NCBI Protein#: NP_000256.4
  • Form of Antibody:  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage:  
  • Store at -20˚C/1 year
  • Immunogen:  
  • Peptide sequence around phosphorylation site of Serine 359 (Q-R-S(p)-K-P) derived from Human p47 phox.
  • reactivity:  
  • Hu
  • appl_detail:  
  • Predicted MW: 45kd
    Western blotting: 1:500~1:1000
  • other_names:  
  • NCF1A, NOXO2, p47phox, SH3PXD1A
  • Purification:  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity:  
  • The antibody detects endogenous level of p47 phox only when phosphorylated at Serine 359.
  • Applications:  
  • WB
  • Background:  
  • NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).



 
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