Na,K-ATPase alpha1 (Phospho-Ser23) Antibody

Catalog Number:

12052

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P05023;
NCBI Gene#: 476;
NCBI Protein#: NP_000692.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 23 (K-K-S(p)-K-K) derived from Rat Na,K-ATPase alpha1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Na,K-ATPase alpha1 only when phosphorylated at Serine 23.

Applications:

WB

Background:

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

References:

appl_detail:

Predicted MW: 113kd
Western blotting: 1:500~1:1000

Datasheet PDF