Product Datasheet  
Na,K-ATPase alpha1 (Phospho-Ser23) Antibody  
Catalog Number: 12052  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species:  
  • Rabbit
  • Amount:  
  • 100μgμg
  • Swiss-Prot No.:  
  • Swiss-Prot#: P05023;
    NCBI Gene#: 476;
    NCBI Protein#: NP_000692.2
  • Form of Antibody:  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage:  
  • Store at -20˚C/1 year
  • Immunogen:  
  • Peptide sequence around phosphorylation site of Serine 23 (K-K-S(p)-K-K) derived from Rat Na,K-ATPase alpha1.
  • reactivity:  
  • Rt
  • appl_detail:  
  • Predicted MW: 113kd
    Western blotting: 1:500~1:1000

  • other_names:  
  • Nkaa1b
  • Purification:  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity:  
  • The antibody detects endogenous level of Na,K-ATPase alpha1 only when phosphorylated at Serine 23.
  • Applications:  
  • WB
  • Background:  
  • This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.




 
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