hnRNP C1/2 (Phospho-Ser260) Antibody

Catalog Number:

12048

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P07910;
NCBI Gene#: 3183;
NCBI Protein#: NP_001070910.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 260 (D-D-S(p)-A-E) derived from Human hnRNP C1/2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of hnRNP C1/2 only when phosphorylated at Serine 260.

Applications:

WB

Background:

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.

References:

appl_detail:

Predicted MW: 41kd
Western blotting: 1:500~1:1000

Datasheet PDF