p27 Kip1 (Phospho-Thr198) Antibody

Catalog Number:

12047

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P46527;
NCBI Gene#: 1027;
NCBI Protein#: NP_004055.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 198 (R-R-R(p)-Q-T) derived from Human p27 Kip1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of p27 Kip1 only when phosphorylated at Threonine 198.

Applications:

WB

Background:

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

References:

appl_detail:

Predicted MW: 27kd
Western blotting: 1:500~1:1000

Datasheet PDF