Product Datasheet  
p27 Kip1 (Phospho-Thr198) Antibody  
Catalog Number: 12047  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P46527;
    NCBI Gene#: 1027;
    NCBI Protein#: NP_004055.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Threonine 198 (R-R-R(p)-Q-T) derived from Human p27 Kip1.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 27kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • KIP1, MEN4, CDKN4, MEN1B, P27KIP1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of p27 Kip1 only when phosphorylated at Threonine 198.
  • Applications：  
  • WB
  • Background：  
  • Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.



 
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