Keratin 8 (Phospho-Ser432) Antibody

Catalog Number:

12042

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P05787;
NCBI Gene#: 3856;
NCBI Protein#: NP_001243211.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 432 (L-T-S(p)-P-G) derived from Human Keratin 8.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Keratin 8 only when phosphorylated at Serine 432.

Applications:

WB

Background:

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

References:

appl_detail:

Predicted MW: 53kd
Western blotting: 1:500~1:1000

Datasheet PDF