BCL-2 (Phospho-Thr69) Antibody

Catalog Number:

12036

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P10415;
NCBI Gene#: 596;
NCBI Protein#: NP_000624.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 69 (A-R-T(p)-S-P) derived from Human BCL-2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of BCL-2 only when phosphorylated at Threonine 69.

Applications:

WB

Background:

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

References:

appl_detail:

Predicted MW: 26kd
Western blotting: 1:500~1:1000

Datasheet PDF