PPAR-BP (Phospho-Thr1457) Antibody

Catalog Number:

12023

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15648;
NCBI Gene#: 5469;
NCBI Protein#: XP_005257523.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 1457 (A-Y-T(p)-P-Q) derived from Human PPAR-BP.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PPAR-BP only when phosphorylated at Threonine 1457.

Applications:

WB

Background:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

References:

appl_detail:

Predicted MW: 168kd
Western blotting: 1:500~1:1000

Datasheet PDF