IkappaB- beta (Phospho-Thr19) Antibody

Catalog Number:

12011

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15653;
NCBI Gene#: 4793;
NCBI Protein#: NP_001230045.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 19 (C-D-S(p)-G-L) derived from Human IkappaB- beta.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of I kappaB- beta only when phosphorylated at Threonine 19.

Applications:

WB

Background:

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

References:

appl_detail:

Predicted MW: 37kd
Western blotting: 1:500~1:1000

Datasheet PDF