HSL (Phospho-Ser552) Antibody

Catalog Number:

12010

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q05469;
NCBI Gene#: 3991;
NCBI Protein#: NP_005348.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 552 (V-L-S(p)-S-L) derived from Human HSL.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of HSL only when phosphorylated at Serine 552.

Applications:

WB

Background:

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

References:

appl_detail:

Predicted MW: 117kd
Western blotting: 1:500~1:1000

Datasheet PDF