Product Datasheet  
HSL (Phospho-Ser552) Antibody  
Catalog Number: 12010  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species:  
  • Rabbit
  • Amount:  
  • 100μgμg
  • Swiss-Prot No.:  
  • Swiss-Prot#: Q05469;
    NCBI Gene#: 3991;
    NCBI Protein#: NP_005348.2
  • Form of Antibody:  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage:  
  • Store at -20˚C/1 year
  • Immunogen:  
  • Peptide sequence around phosphorylation site of serine 552 (V-L-S(p)-S-L) derived from Human HSL.
  • reactivity:  
  • Hu Ms Rt
  • appl_detail:  
  • Predicted MW: 117kd
    Western blotting: 1:500~1:1000

  • other_names:  
  • HSL, LHS
  • Purification:  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity:  
  • The antibody detects endogenous level of HSL only when phosphorylated at Serine 552.
  • Applications:  
  • WB
  • Background:  
  • In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.




 
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