GRF-1 (Phospho-Tyr1105) Antibody

Catalog Number:

12009

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9NRY4;
NCBI Gene#: 2909;
NCBI Protein#: NP_004482.4

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine 1105 (N-I-Y(p)-S-V) derived from Human GRF-1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of GRF-1 only when phosphorylated at Tyrosine 1150.

Applications:

WB

Background:

Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.

References:

appl_detail:

Predicted MW: 155kd
Western blotting: 1:500~1:1000

Datasheet PDF