E2F1 (Phospho-Thr433) Antibody

Catalog Number:

12006

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q01094;
NCBI Gene#: 1869;
NCBI Protein#: NP_005216.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 433 (D-L-T(p)-P-L) derived from Human E2F1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of E2F1 only when phosphorylated at Threonine 433.

Applications:

WB

Background:

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.

References:

appl_detail:

Predicted MW: 47kd
Western blotting: 1:500~1:1000

Datasheet PDF