Product Datasheet  
E2F1 (Phospho-Thr433) Antibody  
Catalog Number: 12006  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q01094;
    NCBI Gene#: 1869;
    NCBI Protein#: NP_005216.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Threonine 433 (D-L-T(p)-P-L) derived from Human E2F1.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 47kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • E2F-1, PBR3, RBAP-1, RBBP-3
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of E2F1 only when phosphorylated at Threonine 433.
  • Applications：  
  • WB
  • Background：  
  • Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.



 
© Signalway Biotechnology All Rights Reserved.