Calmodulin (Phospho-Thr79/Ser81) Antibody

Catalog Number:

11978

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P62158;
NCBI Gene#: 808;
NCBI Protein#: NP_005175.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine79/serine81(K-D-T(p)-D-S(p)-E-E) derived from Human Calmodulin .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Calmodulin only when phosphorylated at threonine 79/serine 81.

Applications:

WB

Background:

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

Allen D, Fakler B, Maylie J, Adelman JP (2007) J Neurosci 27, 2369-76
Arrigoni G, et al. (2004) Biochemistry 43, 12788-98
Greif DM, Sacks DB, Michel T (2004) Proc Natl Acad Sci U S A 101, 1165-70

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF