STAT5A/B (Phospho-Ser725/730) Antibody

Catalog Number:

11977

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P42230/P42232;
NCBI Gene#: 20850/20851;
NCBI Protein#: NP_001275647.1/NP_036580.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine725/730(A-P-S(p)-P-V) derived from Human STAT5A/B.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of STAT5A/B only when phosphorylated at serine 725/ serine 730.

Applications:

WB

Background:

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Ross JA, et al. (2010) J Biol Chem 285, 3582-91
Uddin S, et al. (2003) Biochem Biophys Res Commun 308, 325-30
Xue HH, et al. (2002) Int Immunol 14, 1263-71

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF