n-NOS (Phospho-Ser852) Antibody

Catalog Number:

11975

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P29475;
NCBI Gene#: 4842;
NCBI Protein#: NP_000611.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 852(F-N-S(p)-V-S) derived from Human n-NOS .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of n-NOS only when phosphorylated at serine 852.

Applications:

WB

Background:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Capettini LS, et al. (2011) DBr J Pharmacol 164, 1738-48
Ferreira JM, Burnett AL, Rameau GA (2011) J Neurosci 31, 1991-9
Chaudhury A, Rao YM, Goyal RK (2008) Am J Physiol Gastrointest Liver Physiol 295, G442-51

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF