Vinculin (Phospho-Tyr822) Antibody

Catalog Number:

11973

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P18206;
NCBI Gene#: 7414;
NCBI Protein#: NP_003364.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine 822 (S-G-Y(p)-R-I) derived from Human Vinculin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Vinculin only when phosphorylated at tyrosine 822.

Applications:

WB

Background:

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Subauste MC, et al. (2004) J Cell Biol 165, 371-81
Olsen JV, et al. (2010) Sci Signal 3, ra3
J?rgensen C, et al. (2009) Science 326, 1502-9

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF