Product Datasheet  
Vinculin (Phospho-Tyr822) Antibody  
Catalog Number: 11973  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P18206;
    NCBI Gene#: 7414;
    NCBI Protein#: NP_003364.1
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Tyrosine 822 (S-G-Y(p)-R-I) derived from Human Vinculin.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • Vinculin;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of Vinculin only when phosphorylated at tyrosine 822.
  • Applications：  
  • WB
  • Background：  

  • Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

    Subauste MC, et al. (2004) J Cell Biol 165, 371-81
    Olsen JV, et al. (2010) Sci Signal 3, ra3
    J?rgensen C, et al. (2009) Science 326, 1502-9




 
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