14-3-3 zeta/ delta (Phospho-Thr232) Antibody

Catalog Number:

11954

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P63104;
NCBI Gene#: 7534;
NCBI Protein#: NP_001129171.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine232(S-D-T(p)-Q-G) derived from Human 14-3-3 zeta/ delta .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of 14-3-3 zeta/ delta only when phosphorylated at threonine 232.

Applications:

WB

Background:

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Sluchanko NN, et al. (2011) Arch Biochem Biophys 506, 24-34
Sluchanko NN, et al. (2008) Arch Biochem Biophys 477, 305-12
Clokie SJ, et al. (2005) FEBS J 272, 3767-76

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF